Prediction of protein thermodynamic stability changes upon single-site mutations

PoPMuSiC is a tool for the computer-aided design of mutant proteins with controlled thermodynamic stability properties. It evaluates the changes in folding free energy of a given protein or peptide under point mutations, on the basis of the experimental or modeled protein structure.

Three modes are available:

  1. Systematic: Evaluation of the stability changes resulting from all possible point mutations. Returns a report with the list of the most stabilizing or destabilizing mutations, or of the mutations that do not affect stability.
  2. Manual: Prediction of the stability changes caused by the point mutations specified by the user.
  3. File: Prediction of the stability changes caused by a list of mutations specified by the user in an uploaded file.

PoPMuSiC

Prediction of protein thermostability changes upon single-site mutations

HOTMuSiC is a tool for the computer-aided design of mutant proteins with controlled thermal stability properties. It evaluates the changes in melting temperature of a given protein or peptide under point mutations, on the basis of the experimental or modeled protein structure.

Four modes are available:

  1. Systematic: Evaluation of the stability changes resulting from all possible point mutations. Returns a report with the list of the most stabilizing or destabilizing mutations, or of the mutations that do not affect stability.
  2. Manual: Prediction of the stability changes caused by the point mutations specified by the user.
  3. File: Prediction of the stability changes caused by a list of mutations specified by the user in an uploaded file.
  4. Wild-type Tm: Predictions with improved accuracy are obtained if the Tm of the wild-type protein is known. 

HotMuSiC